Lecture Materials
Unit II: Structure and Function of Biological Molecules
Lecture 4 - Enzymes
Enzymes are biological catalysts; nearly every reaction that takes place in a living cell is catalyzed by an enzyme. Most enzymes are proteins, with some requiring non-protein components called coenzymes in order to function. The control of enzymatic activity plays a central roll in controlling the activities and proper functioning of a living cell.
- Part I - Draft
- PDF with links for viewing
- PDF for printing
- Worked Problems in Lecture
- Example of Lineweaver-Burke analysis of enzyme kinetics to determine Vmax, Km, kcat, and kcat/Km from 6. March, 2018
- Part II - Draft
- Reading Assignments
- Reading: Moran et al. Chapters 5: Properties of Enzymes
- Reading: Moran et al. Chapters 6: Mechanisms of Enzymes
- Reading: Moran et al. Chapters 7: Coenzymes and Vitamins
- We will refer to material in this chapter when specific coenzymes come up in later discussion of enzyme catalzyed reactions.
- Michaelis, L. & Menton M.L., "Die Kinetik der Invertinwirkung" Biochemische Zeitschrift. 1913; 49:333–369.
- Problem Assignments
- Supplemental Questions
- Molecular Models
- Protein Structure - Phosphofructokinase I: Jmol model illustrates an allosteric enzyme.
- Protein Structure - Hexokinase: Jmol model illustrates the concept of "induced fit" upon substrate binding by an enzyme.
- Catalytic Strategies - Chymotrypsin: Jmol illustrates the "catalytic triad", the covalently bound intermediate and the binding interactions between enzyme and substrate.
- Movie
- Links