During glycolysis, gyceraldehyde-3-phosphate dehydrogenase converts glyceraldehyde-3-phosphate (G-3-P) to 1,3-bisphosphoglycerate (1,3-BGP) through oxidative phosphorylation. NAD+ is oxidized to NADH when a Pi is exchanged for a hydrogen on the carbonyl carbon of G-3-P thus converting G-3-P to 1,3-BGP.
Steps of Glyceraldehyde-3-Phosphate Dehydrogenase Reaction Mechanism
 |
I. SH group of cysteine is activated by histidine II. SH attacks CO, H+e- are transferred to NAD III.Thio-ester intermediate IV. Thio-ester attacked by Pi |
Molecular Model of Glyceraldehyde-3-Phosphate Dehydrogenase
|
Glyceraldehyde-3-Phosphate Dehydrogenase is a 160kD protein that functions as a tetramer. Each subunit contains 360 residues and is comprised of two-domains. Four Subunits There are 11 alpha helices and 17 beta sheets in each subunit. Secondary Structure NAD within Glyceraldehyde-3-Phosphate Dehydrogenase Glyceraldehyde-3-Phosphate Dehydrogenase oxidately phosphorylates G-3-P into 1,3-BGP. The acceptor of electrons is NAD+. The reducton of NAD+ proceeds by the enzymatic transfer of a hydride ion from the aldehyde group of G-3-P to the nicotinamide ring of NAD, yielding the reduced coenzyme NADH. NAD+ Binding Display entire structure Use the following commands to adjust the molecular models position. You can also you the mouse to rotate the molecule by clicking on the structure. Reset Position Rotate: Distances: Width (Å) Background Color: White |
|||
