During glycolysis, gyceraldehyde-3-phosphate dehydrogenase converts glyceraldehyde-3-phosphate (G-3-P) to 1,3-bisphosphoglycerate (1,3-BGP) through oxidative phosphorylation. NAD+ is oxidized to NADH when a Pi is exchanged for a hydrogen on the carbonyl carbon of G-3-P thus converting G-3-P to 1,3-BGP.
Steps of Glyceraldehyde-3-Phosphate Dehydrogenase Reaction Mechanism
I. SH group of cysteine is activated by histidine
II. SH attacks CO, H+e- are transferred to NAD
IV. Thio-ester attacked by Pi
Molecular Model of Glyceraldehyde-3-Phosphate Dehydrogenase
Glyceraldehyde-3-Phosphate Dehydrogenase is a 160kD protein that functions as a tetramer. Each subunit contains 360 residues and is comprised of two-domains.
There are 11 alpha helices and 17 beta sheets in each subunit.
NAD within Glyceraldehyde-3-Phosphate Dehydrogenase
Glyceraldehyde-3-Phosphate Dehydrogenase oxidately phosphorylates G-3-P into 1,3-BGP. The acceptor of electrons is NAD+. The reducton of NAD+ proceeds by the enzymatic transfer of a hydride ion from the aldehyde group of G-3-P to the nicotinamide ring of NAD, yielding the reduced coenzyme NADH.
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