Chem 455: Biochemistry Lab II, Spring 2005

Project 2:
Synthesis and Characterization of a Beta-Amyloid Fibril-forming Peptides.

Schedule

30. March, 2005	Overview of Project.
	Read background materials and write draft of Introduction and Procedures section
6. April, 2005	Start Synthesis.
	Setup the Protein Technologies PS-3 Automated Solid Phase Peptide Synthesizer and begin the synthesis.
13. April, 2005	Cleavage and Isolation.
	Cleave the peptide from the resin and isolate it using solvent extractions
20. April, 2005	HPLC analysis and purification of peptides.
	Use HPLC to analyze for peptide purity
27. April, 2005	Structure Analysis.
	Use NMR to look at primary structure
4. May, 2003	Structure Analysis
	Use FTIR to look a secondary structure and to monitor fibril formation
11. May, 2005	Finish up.
20. May, 2005	Final.

Informational
Resources:

This is just a starting point:

PS-3 Automated Solid Phase Peptide Synthesizer User's Guide; Rainin Instrument Co.

Fields, Greg B.; Tian, Zhenping & Barany, George; "Principles and Practice of Solid-Phase Peptide Synthesis;" Synthetic Peptide: A User's Guide; Grant, Gregory A., ed.; Oxford University Press; 1992

Terzi, Evelyne,; Hölzemann, G. & Seelig, Joachim; "Reversible Random Coil-b-Sheet Transition of the Alzheimer b-Amyloid Fragment;" Biochemistry 1994, 33, 1345-1350.

Required
Materials:

Bound notebook and pen for taking lab notes

3-ring binder for handouts.

Safety goggles (They must meet ANSI Z87.1-1989 standards (Available for purchase at the bookstore)

Sharpie‚ pen or wax pencil for marking glassware

Grades

Grades for this project will be based on a written lab report, and a final exam. The lab report will be worth 200 points and the 100 points. The format should follow the instructions to the author given in the journal Biochemistry (http://pubs.acs.org/cgi-bin/dispinst?bichaw).

[Chemistry Dept.] [Chem 455]

Warren Gallagher
Department of Chemistry
(715) 836-5388
wgallagh@uwec.edu

updated: Wednesday, April 6, 2005