The Yeast Two-Hybrid System

3/2/99


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Table of Contents

The Yeast Two-Hybrid System

What is the yeast two-hybrid system used for?

How does it work?

The Model

Steps to Screen a Library

Sequence analysis

Plasmid Constructs

Reporter Genes

Sample Plasmid

Elimination of False Positives

Advantages

Examples of Uses of the Yeast Two-Hybrid System

References

References continued

False Positives

Author: chem

Email: hartsesc@uwec.edu

Home Page: www.chem.uwec.edu/Chem412_S99/